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KMID : 0624620080410050358
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BMB Reports
2008 Volume.41 No. 5 p.358 ~ p.362
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Multiple shRNA expressing vector enhances efficiency of gene silencing
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Song Jun
Giang An
Lu Yingchun
Pang Shen
Chiu Robert
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Abstract
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RNA interference (RNAi) is the process of sequence-specific gene silencing. However, RNAi efficiency still needs to be improved for effective inhibition of target genes. We have developed an effective strategy to express multiple shRNAs (small hairpin RNA) simultaneously using multiple RNA Polymerase III (Pol III) promoters in a single vector. Our data demonstrate that multiple shRNAs expressed from Pol III promoters have a synergistic effect in repressing the target gene. Silencing of endogenous cyclophilin A (CypA) or key HIV viral genes by multiple shRNAs results in significant inhibition of the target gene. [BMB reports 2008; 41(5): 358-362]
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KEYWORD
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Gene silencing, RNA interference, RNAi, shRNA, Vector
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